Lentivirus vector with controllable expression of gene of interest, and packaging method therefor

Abstract

The present application relates to a lentivirus vector with controllable expression of a gene of interest, and a packaging method therefor. The lentivirus packaging vector contains a first LTR, a reversely inserted gene expression cassette and a second LTR, wherein the first LTR is positioned upstream of the reversely inserted gene expression cassette in the direction of viral genome expression; the second LTR is positioned downstream of the reversely inserted gene expression cassette in the direction of viral genome expression; the gene expression cassette contains a promoter, a repressible operon and an optional gene of interest, which are connected in sequence; and in the presence of a repressor, the repressible operon is capable of repressing the expression of the gene of interest positioned downstream thereof.

Dependencies are inferred from the claim text.