Mixed mode chromatographic packing material
OwnerDovile Baziulyte-Paulaviciene, Matas Damonskis +2inventors
InventorsDovile Baziulyte-Paulaviciene, Matas Damonskis, Richard Thomas Williams, Martinš Jansons
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Abstract
The present invention relates to the field of chromatographic sample separation that includes liquid chromatography and solid phase extraction and, in particular, it relates to material and the synthesis of material for use as a stationary phase in chromatographic sample separation. The invention further relates to uses of the material, in particular in the separation of hydrophilic and hydrophobic peptides, non-glycosylated and N-linked glycosylated peptides, deamidated and oxidized peptides. The invention also relates to chromatographic columns and solid phase extraction columns containing the material as a stationary phase.
Claims28
A method for making chromatographic packing material, wherein the packing material comprises a functionalised silicone-based polymer layer bonded to substrate particles and the method for forming the packing material comprises:
- (a) (i) forming silicone-based polymer encapsulated substrate particles comprising pendant functional groups by reacting functional groups on the substrate particles with a silicone-based polymer product comprising pendant functional groups;
- and
- (ii) functionalising the silicone-based polymer encapsulated substrate particles by reacting the pendant functional groups on the silicone-based polymer encapsulated substrate particles with at least one hydrophobic compound and at least one amine containing compound;
- or
- (b) (i) forming a functionalised silicone-based polymer by reacting pendant functional groups on a silicone-based polymer product with at least one hydrophobic compound and at least one amine containing compound;
- and
- (ii) reacting the product of step b (i) with functional groups on the substrate particles.
A chromatographic packing material comprising:
- (i) Substrate particles;
- (ii) A functionalised silicone-based polymer bonded to the substrate particles prepared by:
- (a) (i) forming silicone-based polymer encapsulated substrate particles comprising pendant functional groups by reacting functional groups on the substrate particles with a silicone-based polymer product comprising pendant functional groups;
- and
- (ii) functionalising the silicone-based polymer encapsulated substrate particles by reacting the pendant functional groups on the silicone-based polymer encapsulated substrate particles with at least one hydrophobic compound and at least one amine containing compound;
- or
- (b) (i) forming a functionalised silicone-based polymer by reacting pendant functional groups on a silicone-based polymer product with at least one hydrophobic compound and at least one amine containing compound;
- and
- (ii) reacting the product of step b (i) with functional groups on the substrate particles.
Dependencies are inferred from the claim text.