Genetically engineered innate lymphoid cells for enhancing lifespan and/or treating cancers
AssigneeAcademia Sinica
InventorsChe-Kun James SHEN, Chun-Hao HUNG, Shih-Ling HUANG, Keh-Yang WANG, Jing-Ping WANG, Zheng-Sheng LAI
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Abstract
The disclosure is related to the field of innate lymphoid cells (ILCs). Particularly, the disclosure pertains to genetically engineered NK cells carrying a modified Eklf gene encoding a modified EKLF polypeptide.
Claims20
A method of increasing longevity and/or treating cancer in a subject, comprising administering an effective amount of genetically engineered innate lymphoid cells to the subject, wherein the genetically engineered innate lymphoid cells express a modified EKLF polypeptide comprising an amino acid substitution at a sumoylation site of a wild-type EKLF polypeptide having the amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2.
An in vitro method of producing genetically engineered innate lymphoid cells, comprising:
- (a) providing a transgenic animal expressing a modified human EKLF polypeptide comprising an amino acid substitution at the sumoylation site that corresponds to lysine at position 54 of the wild type human EKLF having the amino acid sequence of SEQ ID NO:
- 1, or a modified mouse EKLF polypeptide comprising an amino acid substitution at the sumoylation site that corresponds to lysine at position 74 of the wild type mouse EKLF having the amino acid sequence of SEQ ID NO:2;
- and
- (b) isolating innate lymphoid cells from the genetically engineered animal.
An engineered innate lymphoid cell comprises a gene encoding a modified EKLF polypeptide, wherein the modified EKLF polypeptide comprises an amino acid substitution at a sumoylation site of a wild-type EKLF polypeptide, wherein the sumoylation site corresponds to lysine at position 54 of a wild type human EKLF having the amino acid sequence of SEQ ID NO:
- 1, or corresponds to lysine at position 74 of a wild type mouse EKLF having the amino acid sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.