Strain of microorganisms and process for the fermentative production of gamma-glutamyltyrosine and gamma-glutamylphenylalanine
AssigneeWacker Chemie AG
InventorsThomas Schlösser, Marcel Thön
Life of this patent
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Abstract
A micro-organism strain and process for fermentative production of the same. The fermentative production of the substances gamma-glutamyltyrosine and gamma-glutamylphenylalanine by a micro-organism strain which is cultured in a fermentation medium. The fermentation medium is removed from the micro-organisms after the fermentation and gamma-glutamyltyrosine and gamma-glutamylphenylalanine are isolated from the fermentation medium. The microorganism strain contains at least one further gene encoding a glutamate-cysteine ligase and is deficient in glutathione synthetase. For the culture, the fermentation medium is admixed with L-tyrosine and L-phenylalanine.
Claims6
1-7. (canceled)
A process for fermentative production of gamma-glutamyltyrosine and gamma-glutamylphenylalanine:
- comprising:
- providing a bacterial strain of the Enterobacteriaceae family that is cultured in a fermentation medium, wherein the fermentation medium is removed from the cells after the fermentation, and gamma-glutamyltyrosine and gamma-glutamylphenylalanine are isolated from the fermentation medium,
- wherein the bacterial strain
- a) either contains an open reading frame encoded in the wild-type genome that encodes glutamate-cysteine ligase and, in addition, at least one further open reading frame (ORF) encoding a glutamate-cysteine ligase, or
- b) contains no ORF encoded in the wild-type genome that encodes a glutamate-cysteine ligase, but at least one further ORF encoding a glutamate-cysteine ligase;
- wherein the bacterial strain is deficient in glutathione synthetase, i.e., no functional glutathione synthetase is produced by the corresponding gene;
- and
- wherein the fermentation medium contains at least 50 mg/L L-tyrosine and 50 mg/L L-phenylalanine.
Dependencies are inferred from the claim text.