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US 2025/0270293 A1Published application
Scaffold proteins
AssigneeAvacta Life Sciences Limited
InventorsGeoffrey William Platt, Graham Robert Pye-Smith Spence
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Abstract
The invention relates to a polypeptide, such as an Affimer polypeptide, comprising an amino acid sequence having at least 80% identity to amino acid residues 1 to 11, 13 to 15, 17 to 19, 21 to 25, 27 to 28, 35 to 37, 39, 41, 43 to 44, 46 to 47, 49 to 50, 52 to 53, 55 to 58, 63 to 64, 66, 68 to 82, 84 to 85, and 87 to 98 of SEQ ID NO: 1; characterized in that said polypeptide comprises one or mutations relative to SEQ ID NO: 1. The invention also relates to various methods and nucleic acids.
Claims18
-42. (canceled)
A conjugate comprising:
- (i) a polypeptide comprising a scaffold protein sequence, wherein the scaffold protein sequence comprises:
- (a) a N32G mutation relative to the amino acid sequence of SEQ ID NO:
- 1;
- (b) at least 80% identity to the amino acid sequence of SEQ ID NO:
- 1;
- (c) a first heterologous peptide inserted between positions 48 and 50, relative to SEQ ID NO:
- 1, wherein the first heterologous peptide has a length of 3 to 20 amino acids;
- and
- (d) a second heterologous peptide inserted between positions 73 and 78, relative to SEQ ID NO:
- 1, wherein the second heterologous peptide has a length of 3 to 20 amino acids;
- wherein the polypeptide has increased thermostability relative to SEQ ID NO:1, and the first heterologous peptide and second heterologous peptides are excluded when determining the at least 80% identity;
- (ii) a cleavable linker;
- and
- (iii) a therapeutic agent.
11 more elements
Dependencies are inferred from the claim text.