Als-inhibitor herbicide tolerant beta vulgaris hybrids with increased heterosis
OwnerMaik GERTZ, Martin MESSERSCHMIDT +1inventors
InventorsMaik GERTZ, Martin MESSERSCHMIDT, Ottó TÖRJÉK
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Abstract
Provided are improved herbicide tolerant Beta vulgaris hybrid plants and parts thereof, particularly improved hybrid sugar beet or fodder beet plants, with increased yield performance, while maintaining optimal and agronomically relevant herbicide tolerance, wherein both parent plants are derived by introgression of the herbicide tolerance gene from a single herbicide resistance donor genotype. Further provided are Beta vulgaris parent plants useful for obtaining such hybrid plants, markers for identifying such improved herbicide tolerant Beta vulgaris plants, as well methods for obtaining and identifying such improved herbicide tolerant Beta vulgaris hybrid plants.
Claims20
A Beta vulgaris hybrid plant, or hybrid seed, or a part thereof, comprising an ALS-herbicide tolerant endogenous ALS gene allele which is homozygously present on chromosome 5 in said Beta vulgaris hybrid plant or seed, said ALS-herbicide tolerant endogenous ALS gene allele encoding an ALS protein comprising leucine at position 569, said Beta vulgaris hybrid plant or seed being obtainable by crossing two parent Beta vulgaris plants from different heterotic pools, wherein each parent plant comprises the ALS-herbicide tolerant endogenous ALS gene allele in homozygous state and wherein the ALS-herbicide tolerant endogenous ALS gene allele in each parent plant is introgressed from the same ALS-herbicide tolerant endogenous ALS gene allele donor plant, characterized in that chromosomal region of chromosome 5 introgressed from said ALS-herbicide tolerant endogenous ALS gene allele donor plant and located upstream and/or downstream of the ALS inhibitor tolerant endogenous ALS gene allele in said parent plant is sufficiently small to avoid or decrease inbred depression and/or increase heterosis and/or increase sugar yield in said Beta vulgaris hybrid plant.
A DNA molecule consisting of the chromosomal region of chromosome 5 of a Beta vulgaris ALS inhibitor herbicide tolerant plant comprising an ALS-herbicide tolerant endogenous ALS gene, reference seed of which has been deposited as NCIMB 41705, which is located on a chromosomal interval flanked on one side of the ALS-herbicide tolerant endogenous ALS gene allele by a marker selected from the group consisting of marker M1, marker M2, marker M3, marker M4, marker M11, marker M15, marker M14 and marker M5, and on the other side of the ALS-herbicide tolerant endogenous ALS gene allele is flanked by a marker selected from the group consisting of marker M6, marker M7, marker M8, marker M9 and marker M10, marker M12, marker M13, marker M16 and marker M17.
A method for identifying a genomic fragment of chromosome 5 in (elite) a Beta vulgaris plant, introgressed from an ALS-herbicide tolerant Beta vulgaris donor plant, said genomic fragment comprising an ALS-herbicide tolerant endogenous ALS gene allele, or for identifying/selecting a Beta vulgaris plant comprising said genomic fragment of chromosome 5, comprising the steps of
- a. identifying the presence of the ALS-herbicide tolerant endogenous ALS gene allele in said plant by a phenotypic or a marker-assisted method;
- and
- b. identifying the presence of at least one allele/nucleotide on a chromosomal interval flanked on one side of the ALS-herbicide tolerant endogenous ALS gene allele by a marker selected from the group consisting of marker M1 (comprising a nucleotide sequence of SEQ ID NO. 1), marker M2 (comprising a nucleotide sequence of SEQ ID NO. 2), marker M5 (comprising a nucleotide sequence of SEQ ID NO. 5), marker M11 (comprising a nucleotide sequence of SEQ ID NO. 11), marker M14 (comprising a nucleotide sequence of SEQ ID NO. 19) and marker M15 (comprising a nucleotide sequence of SEQ ID NO. 20), and on the other side of the ALS-herbicide tolerant endogenous ALS gene allele is flanked by a marker selected from the group consisting of marker M6 (comprising a nucleotide sequence of SEQ ID NO. 6), marker M12 (comprising a nucleotide sequence of SEQ ID NO. 12), marker M13 (comprising a nucleotide sequence of SEQ ID NO. 13), marker M7 (comprising a nucleotide sequence of SEQ ID NO. 7), marker M8 (comprising a nucleotide sequence of SEQ ID NO. 8), marker M9 (comprising a nucleotide sequence of SEQ ID NO. 9), marker M10 (comprising a nucleotide sequence of SEQ ID NO. 10), marker M16 (comprising a nucleotide sequence of SEQ ID NO. 21) and marker M17 (comprising a nucleotide sequence of SEQ ID NO. 22);
- and
- c. optionally selecting an ALS inhibitor tolerant Beta vulgaris plant, wherein said plant exhibits a decreased inbred depression and/or an increased heterosis and/or an increased sugar yield or biomass yield.
A DNA molecule comprising the nucleotide sequence of any one of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, or SEQ ID NO. 17.
Dependencies are inferred from the claim text.