US 2025/0268162 A1 · POLYPLOID HYBRID POTATO BREEDING

Abstract

The present inventions relate to a breeding system for the production of polyploid potato seeds, plants, or plant parts where cycles of meiosis, syngamy, and selection are used for interpopulation improvement of progenitor lines, and sexual polyploidization occurs during hybrid production by inducing clonal gamete formation in the parents that are to be crossed. Reciprocal recurrent selection can be used to inform selection of candidate potato lines that are either advanced to a gene editing or genetic modification system or crossed and selected to induce clonal gamete formation by arresting meiotic recombination and chromosome reduction. Crosses of parent plants bearing clonal gametes are planned and executed based upon predicted heterotic performance at the polyploid level. The final product is a homogeneous population of hybrid polyploid potato seed, or derivative thereof, bearing both parents' complete nuclear genomes. In some instances, the method is used to generate seedless plants.

Claims30

Dependent claims

A population of polyploid potato seed comprising a subpopulation of genetically uniform polyploid potato seed in an amount of at least 50% of the total number of seeds, the genetically uniform polyploid potato seed comprising three or more haplotypes, wherein the population was obtained from a single potato plant or a set of F1 hybrid potato plants, wherein the subpopulation of genetically uniform polyploid potato seed comprise (A) (1) a germplasm genetic modification means for inhibiting sister chromatid cohesion during the first division of meiosis, (2) a germplasm genetic modification means for inhibiting DNA double strand breakage during meiotic recombination, and (3) a germplasm genetic modification means for inhibiting progression through the second division of meiosis;

or (B) (2) a germplasm genetic modification means for preventing DNA double strand breakage during meiotic recombination, and (4) a germplasm genetic modification means for preventing progression through the first division of meiosis;
wherein each of the germplasm genetic modifications are (i) a complete set that achieves the inhibition, (ii) a partial set that does not achieve inhibition, or (iii) partially complemented at one or more of the genetic modification means and achieve sterility.

A method of breeding a polyploid hybrid potato line, the method comprising:

(a) obtaining a set of potato lines;
(b) breeding the potato lines using traditional plant breeding methods to produce a set of candidate potato lines;
(c) selecting two or more candidate potato lines together comprising three or more haplotypes;
(d) generating a first parent MiMe potato plant and a second parent MiMe potato plant from the two or more candidate potato lines that together comprise the three or more haplotypes;
(e) providing clonal gametes from each of the first and second parent MiMe potato plants;
(f) crossing the clonal gametes to produce a hybrid polyploid potato seed comprising the three or more haplotypes;
(g) growing the hybrid polyploid potato seed to produce a hybrid polyploid potato plant comprising three or more haplotypes;
and
(h) evaluating one or more characteristics of the hybrid polyploid potato plant.

6 more elements

A method of breeding a polyploid hybrid potato line, the method comprising:

(a) obtaining a set of potato lines;
(b) breeding the potato lines using traditional plant breeding methods to produce a set of candidate potato lines;
(c) selecting two or more candidate potato lines together comprising three or more haplotypes;
(d) generating a first parent MiMe potato plant from one of the two or more candidate potato lines;
(e) providing clonal gametes from the first parent MiMe potato plant;
(f) providing haploid gametes from a homozygous parent non-MiMe potato plant of one of the two or more candidate potato lines;
(g) crossing the clonal gametes with the haploid gametes to produce a hybrid polyploid potato seed;
(h) growing the hybrid polyploid potato seed to produce a hybrid polyploid potato plant;
and
(i) evaluating one or more characteristics of the hybrid polyploid potato plant,
wherein the first parent MiMe potato plant and the homozygous parent non-MiMe potato plant together comprise three or more haplotypes, wherein the crossing of step (g) results in the hybrid polyploid potato seed comprising three or more haplotypes, and wherein the growing of step (h) results in the hybrid polyploid potato plant comprising three or more haplotypes.

8 more elements

A genetically modified potato plant, plant part, or plant cell comprising:

(A) three or more haplotypes;
and
(B)
(i) a complete MiMe genotype comprising MiMe alleles conferring decreased expression of one or more MiMe loci of each of a first, second, and third MiMe component, wherein the first MiMe component, the second MiMe component, and the third MiMe component are selected from the group consisting of (1) a component of sister chromatid cohesion during the first division of meiosis, (2) a component of DNA double strand breakage during meiotic recombination, and (3) a component of progression through the second division of meiosis, and each of the first MiMe component, the second MiMe component, and the third MiMe component are different MiMe components;
ii) a partial MiMe genotype comprising:
(a) one or more MiMe alleles conferring decreased expression of one or more MiMe loci of each of a first, second, and third MiMe component;
and
(b) one or more non-MiMe alleles at the one or more MiMe loci of each of the first, second, and third MiMe component,
wherein the first MiMe component, the second MiMe component, and the third MiMe component are selected from the group consisting of (1) a component of sister chromatid cohesion during the first division of meiosis, (2) a component of DNA double strand breakage during meiotic recombination, and (3) a component of progression through the second division of meiosis, and each of the first MiMe component, the second MiMe component, and the third MiMe component are different MiMe components;
iii) a complete MiMe genotype comprising MiMe alleles conferring decreased expression of one or more MiMe loci of each of a first and second MiMe component, wherein the first MiMe component and the second MiMe component are selected from the group consisting of (2) a component of DNA double strand breakage during meiotic recombination and (4) a component of progression through the first division of meiosis, and each of the first MiMe component and the second MiMe component are different MiMe components;
iv) a partial MiMe genotype comprising:
(a) one or more MiMe alleles conferring decreased expression of one or more MiMe loci of each of the first and second MiMe component;
and
(b) one or more non-MiMe alleles at the one or more MiMe loci of each of the first and second MiMe component,
wherein the first MiMe component and the second MiMe component are selected from the group consisting of (2) a component of DNA double strand breakage during meiotic recombination and (4) a component of progression through the first division of meiosis and each of the first MiMe component and the second MiMe component are different MiMe components;
v) a partially complemented MiMe genotype comprising:
(a) only MiMe alleles at one or more MiMe loci of a first MiMe component;
(b) one or more MiMe alleles and one or more non-MiMe alleles at a first MiMe locus of a second MiMe component, and one or more MiMe alleles and one or more non-MiMe alleles at a second MiMe locus of the second MiMe component;
and
(c) either (i) only MiMe alleles at one or more MiMe loci of a third MiMe component, or (ii) one or more MiMe alleles and one or more non-MiMe alleles at a first MiMe locus of the third MiMe component, and one or more MiMe alleles and one or more non-MiMe alleles at a second MiMe locus of the third MiMe component,
wherein the first MiMe component, the second MiMe component, and the third MiMe component are selected from the group consisting of (1) a component of sister chromatid cohesion during the first division of meiosis, (2) a component of DNA double strand breakage during meiotic recombination, and (3) a component of progression through the second division of meiosis, and each of the first MiMe component, the second MiMe component, and the third MiMe component are different MiMe components;
vi) a partially complemented MiMe genotype comprising:
(a) only MiMe alleles at one or more MiMe loci of a first MiMe component;
and
(b) one or more MiMe alleles and one or more non-MiMe alleles at a first MiMe locus of a second MiMe component, and one or more MiMe alleles and one or more non-MiMe alleles at a second MiMe locus of the second MiMe component,
wherein the first MiMe component and the second MiMe component are selected from the group consisting of (2) a component of DNA double strand breakage during meiotic recombination and (4) a component of progression through the first division of meiosis and each of the first MiMe component and the second MiMe component are different MiMe components;
or
vii) a partially complemented MiMe genotype comprising:
(a) only MiMe alleles at one or more MiMe loci of a first MiMe component, wherein the first MiMe component is a component of DNA double strand breakage during meiotic recombination;
(b) one or more MiMe alleles and one or more non-MiMe alleles at one or more MiMe loci of a second MiMe component;
(c) one or more MiMe alleles and one or more non-MiMe alleles at one or more MiMe loci of a third MiMe component;
and
(d) one or more MiMe alleles and one or more non-MiMe alleles at one or more MiMe loci of a fourth MiMe component,
wherein the second MiMe component, the third MiMe component, and the fourth MiMe component are selected from the group consisting of (1) a component of sister chromatid cohesion during the first division of meiosis, (3) a component of progression through the second division of meiosis, and (4) a component of progression through the first division of meiosis, and each of the second MiMe component, the third MiMe component, and the fourth MiMe component are different MiMe components,
wherein (1) the one or more MiMe loci of the component of sister chromatid cohesion during the first division of meiosis comprise REC8, SWITCH1/DYAD, or a combination thereof;
(2) the one or more MiMe loci of the component of DNA double strand breakage during meiotic recombination comprise PAIR1, SPO11-1, SPO11-2, PRD1, PRD2, DFO, MTOPVIB, DSY1, SY1, SY2, SY3, SY4, or any combination thereof;
(3) the one or more MiMe loci of the component of progression through the second division of meiosis comprise OSD1, CYCA1, TDM1, PC1, PC2, FC, or any combination thereof;
and/or
(4) the one or more MiMe loci of the component of progression through the first division of meiosis comprise PS1, JASON, or a combination thereof.

36 more elements

Dependencies are inferred from the claim text.

Polyploid hybrid potato breeding

Life of this patent

Polyploid hybrid potato breeding

Life of this patent

Abstract

Claims30