Regulatory t cell stability assay
AssigneeAbata Therapeutics, Inc.
InventorsYanbo ZHANG, Devapregasan MOODLEY
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Abstract
The present disclosure is directed, in some aspects to methods and compositions for the assessment of regulatory T cell stability over time. In other aspects, the present disclosure provides methods and compositions for expanding regulatory T cells in culture.
Claims71
A method of evaluating the stability profile of an isolated cell population comprising regulatory T cells, the method comprising:
- (i) culturing the isolated cell population in the presence of two or more pro-inflammatory cytokines for a period of time;
- (ii) characterizing one or more markers of stability of the isolated cell population following the period of time;
- and
- (iii) evaluating the stability profile of the isolated cell population based on the characterizing of the one or more markers of stability.
A method comprising:
- (i) culturing an isolated cell population comprising regulatory T cells in the presence of two or more pro-inflammatory cytokines for a period of time;
- and
- (ii) characterizing one or more markers of stability of the isolated cell population following the period of time.
The method of 3, wherein the concentration of IL-2 is in the range of 1-10000 U/mL, 1-7500 U/mL, 1-5000 U/mL, 1-2500 U/mL, 1-1000 U/mL, 100-5000 U/mL, 100-2500 U/mL, 100-1000 U/mL, 100-500 U/mL, 250-1000 U/mL, 250-2500 U/mL, 500-5000 U/mL, 500-2500 U/mL, 500-1000 U/mL, or 750-2000 U/mL.
A method of expanding a cell population, the method comprising culturing an isolated cell population comprising regulatory T cells in the presence of IL-2 and at least three additional pro-inflammatory cytokines.
A method of expanding a cell population, the method comprising culturing an isolated cell population comprising regulatory T cells in the presence of Interleukin-17 (IL-17A).
A method of expanding a cell population, the method comprising culturing an isolated cell population comprising regulatory T cells in the presence of Interferon beta (IFN-β).
A method of expanding a cell population, the method comprising culturing an isolated cell population comprising regulatory T cells in the presence of Interferon gamma (IFN-γ).
A method of expanding a cell population, the method comprising culturing an isolated cell population comprising regulatory T cells in the presence of Interleukin-1b (IL-1β).
A method of expanding a cell population, the method comprising culturing an isolated cell population comprising regulatory T cells in the presence of Tumor necrosis factor alpha (TNF-α) and IL-2.
Dependencies are inferred from the claim text.