Addressing light absorbance during interferometric testing through algorithmic deconvolution and computation

A method implemented by a computer program executing on an interferometric sensing system that measures binding of analyte molecules in a liquid sample to a probe, the method comprising:

• obtaining a dataset that is representative of a sequential order of signals,
• wherein each signal is representative of a series of values indicative of intensity of light across a series of wavelengths at a corresponding point in time, the light being received from the probe that is suspended in the liquid sample;
• for each of the signals,
• producing a subtraction curve by comparing the corresponding series of values to another series of values associated with a reference signal;
• calculating an absorbance ratio based on an analysis of the subtraction curve;
• determining whether a principal component of that signal is reflection or absorption based on the absorbance ratio;
• in response to a determination that the principal component is reflection,
• computing a binding magnitude so as to have an absolute value of that signal as the binding magnitude;
• in response to a determination that the principal component is absorption,
• computing a binding magnitude based on an asymmetric component of the subtraction curve;
• and
• causing display of the binding magnitudes computed for the signals on a plot that is viewable on the interferometric sensing system.