Novel markers combination in sweet basil for disease resistances

A method for producing a disease resistant cultivated basil plant, the method comprising:

• a. selecting a first Ocimum americanum basil plant for having a first allele conferring basil downy mildew (BDM) race-0 resistance, wherein the first allele comprises a nucleotide sequence of SEQ ID NO:1 with a Thymine (T) at position 7, a nucleotide sequence of SEQ ID NO:2 with an Adenine (A) at position 5, a nucleotide sequence of SEQ ID NO:3 with a Cytosine (C) at position 48, a nucleotide sequence of SEQ ID NO:
• 4 with a Guanine (G) at position 4, a nucleotide sequence of SEQ ID NO:5 with a T at position 19, a nucleotide sequence of SEQ ID NO:6 with an A at position 8, and a nucleotide sequence of SEQ ID NO:7 with a T at position 29 resistance;
• and
• selecting a second Ocimum americanum basil plant for having a second allele conferring basil downy mildew (BDM) race-1 resistance, wherein the second allele comprises a nucleotide sequence of SEQ ID NO:8 with a T at position 55, a nucleotide sequence of SEQ ID NO:9 with a T at position 41, a nucleotide sequence of SEQ ID NO:
• 10 with a C at position 11, and a nucleotide sequence of SNP of SEQ ID NO:11 with a G at position 6;
• b. crossing the first BDM race-O resistant Ocimum americanum basil plant and the second BDM race-1 resistant Ocimum americanum basil plant with a BDM nonresistant Ocimum basilicum basil plant to produce a first and a second F1 plant, respectively;
• c. backcrossing and optionally self-pollinating the first F1 plant, and selecting for a first BDM race-0 resistant Ocimum basilicum basil plant comprising the first allele conferring BDM race-0 resistance, and
• backcrossing and optionally self-pollinating the second F1 plant, and selecting for a second BDM race-1 resistant Ocimum basilicum basil plant comprising the second allele conferring BDM race-1 resistance;
• d. crossing the first and second Ocimum basilicum basil plants to produce a double BDM race-0 and race-1 resistant Ocimum basilicum basil plant, optionally backcrossing the F1 plants from the cross with a BDM nonresistant basil plant;
• and
• e. selecting for Ocimum basilicum basil plant having resistance to BDM race-0 and BDM race-1, wherein the double resistant basil plant has introgressed into its genome the first and the second alleles, comprising the nucleotide sequence of SEQ ID NO:1 with a Thymine (T) at position 7, the nucleotide sequence of SEQ ID NO:2 with an Adenine (A) at position 5, the nucleotide sequence of SEQ ID NO:3 with a Cytosine (C) at position 48, the nucleotide sequence of SEQ ID NO:4 with a Guanine (G) at position 4, the nucleotide sequence of SEQ ID NO:5 with a T at position 19, the nucleotide sequence of SEQ ID NO:6 with an A at position 8, the nucleotide sequence of SEQ ID NO:
• 7 with a T at position 29 resistance, the nucleotide sequence of SEQ ID NO:8 with a T at position 55, the nucleotide sequence of SEQ ID NO:9 with a T at position 41, the nucleotide sequence of SEQ ID NO:
• 10 with a C at position 11, and the nucleotide sequence of SNP of SEQ ID NO:11 with a G at position 6, conferring an increased resistance to BDM race-0 and to BDM race-1, as compared to that of a basil plant comprising only the first allele or comprising only the second allele.