Methods and materials for assessing loss of heterozygosity
AssigneeMyriad Genetics, Inc.
InventorsVictor Abkevich, Alexander Gutin, Kirsten Timms, Jerry Lanchbury
Life of this patent
Estimating the term…
Abstract
This document provides methods and materials involved in assessing samples (e.g., cancer cells) for the presence of a loss of heterozygosity (LOH) signature. For example, methods and materials for determining whether or not a cell (e.g., a cancer cell) contains an LOH signature are provided. Materials and methods for identifying cells (e.g., cancer cells) having a deficiency in homology directed repair (HDR) as well as materials and methods for identifying cancer patients likely to respond to a particular cancer treatment regimen also are provided.
Claims29
A method for detecting at least one indicator loss of heterozygosity (LOH) region in genomic DNA, comprising:
- (a) contacting genomic DNA from a cancer cell obtained from a subject with a single nucleotide polymorphism (SNP) array, wherein the at least one pair of human chromosomes are not a human X/Y sex chromosome pair, and wherein the cancer cell is selected from the group consisting of a breast cancer cells an ovarian cancer cell, a leukemia cancer cell, an esophageal cancer cell, a lung cancer cell, and a prostate cancer cell;
- (b) genotyping a plurality of loci from at least one pair of human chromosomes in by contacting the genomic DNA;
- and
- (c) detecting an indicator LOH region in the genomic DNA based on homozygosity of the genotypes of the plurality of loci, wherein the indicator LOH region is longer than 1.5 megabases but shorter than the length of the whole chromosome containing the LOH region.
A method for detecting at least one indicator loss of heterozygosity (LOH) region in genomic DNA, comprising:
- (a) sequencing genomic DNA from a cancer cell obtained from a subject, wherein the at least one pair of human chromosomes are not a human X/Y sex chromosome pair, and wherein the cancer cell is selected from the group consisting of a breast cancer cells an ovarian cancer cell, a leukemia cancer cell, an esophageal cancer cell, a lung cancer cell, and a prostate cancer cell;
- (b) genotyping a plurality of loci from at least one pair of human chromosomes in by contacting the genomic DNA;
- and
- (c) detecting an indicator LOH region in the genomic DNA based on homozygosity of the genotypes of the plurality of loci, wherein the indicator LOH region is longer than 1.5 megabases but shorter than the length of the whole chromosome containing the LOH region.
Dependencies are inferred from the claim text.