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AssigneeOnegene Biotechnology Inc.
InventorsSungjin Park, Daeseong Im, Ryuryun Kim, Minsum Kim, Jaeyoung Choi
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The present invention provides a multifunctional multispecific multimeric biomolecule polymer which is formed by obtaining a biomolecule, to which a ubiquitin C-terminal tag is bound, by recombinantly expressing the biomolecule from a host cell, and polyubiquitinating, in vitro, the biomolecule along with a substrate, and proteins E1 (activation enzyme), E2 (conjugation enzyme) and E3 (ligase) which are involved in ubiquitination, and thus having the biomolecule bind to a polyubiquitin scaffold which is formed by covalently bonding two or more ubiquitins. The biomolecule of the present invention may be one or more selected from the group consisting of a protein, peptide, polypeptide, antibody, antibody fragment, DNA and RNA, and, for example, by using heterologous proteins, modularized functionality may be imparted to the multifunctional multispecific biomolecule polymer. In addition, according to the present invention, the multifunctional multispecific multimeric biomolecule polymer is provided in a form that is bound to a molecule capable of increasing the in vivo duration, and thus may be used for producing drugs requiring the increased in vivo duration of efficacy.
A multifunctional multispecific multimeric biomolecule polymer, comprising a polyubiquitin scaffold which is formed by covalently bonding two or more ubiquitins, and 2 to 10 biomolecules comprising binding moieties, each specific for different binding sites, wherein the biomolecule comprises active sites that specifically bind to other biomolecules, small molecule chemical compounds or nanoparticles, and is directly bound to the N-terminus, the C-terminus, or both the N-terminus and the C-terminus of the ubiquitin or is bound by a linker, and a carrier that prolongs the in vivo stability and duration of the biomolecule is directly bound to the N-terminus, the C-terminus, or both the N-terminus and the C-terminus of the ubiquitin or is bound by a linker.
A method for preparing a multifunctional multispecific multimeric biomolecule polymer, in which a polyubiquitin scaffold, two or more biomolecules comprising binding moieties, each specific for different binding sites, and a carrier that prolongs the in vivo duration are directly bound to the N-terminus or the C-terminus of the ubiquitin or are bound by a linker, wherein the method comprises
Dependencies are inferred from the claim text.