Non-immunogenic tissue augmentation formulation and method for fabrication
AssigneeBoard of Trustees of Michigan State University
InventorsMorteza MAHMOUDI, Shahriar SHARIFI
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Abstract
Disclosed herein are tissue augmentation compositions comprising a hydrogel modified with methacrylic anhydride. The hydrogel may be composed of albumin, collagen, silk, or keratin. The composition may be used to modulate an immune response for tissue augmentation and/or as a dermal filler. The composition is non-immunogenic, non-toxic, and may be used for various biomedical applications, including passivation coatings for biomedical devices, depots, and inserts.
Claims41
A hydrogel composition comprising a methacrylated protein, wherein the degree of methacryloylation of the methacrylated protein is greater than about 85%.
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A method for modulating an immune response in a subject comprising administering the hydrogel composition a subject in need thereof the hydrogel composition comprising a methacrylated protein, wherein the degree of methacryloylation of the methacrylated protein is greater than about 85%.
A hydrogel composition comprising a methacrylated protein prepared by
- dissolving an unmethacrylated protein in a buffered solution;
- adding methacrylic anhydride to the buffered solution to prepare a reaction mixture;
- incubating the reaction mixture to generate the methacrylated protein;
- isolating the methacrylated protein;
- freeze-drying the methacrylated protein;
- dissolving the freeze-dried methacrylated protein in deionized water to prepare a hydrogel precursor solution;
- adding a photoinitiator to the hydrogel precursor solution and mixing;
- and
- exposing the hydrogel precursor solution to ultraviolet light to cross-link the methacrylated protein to prepare the hydrogel composition,
- wherein the ultraviolet intensity is between about 1 and about 20 mW/cm2.
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Dependencies are inferred from the claim text.