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AssigneeCellscript, LLC
InventorsJudith MEIS, Anthony PERSON, Cynthia CHIN, Jerome JENDRISAK, Gary DAHL
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The present invention relates to compositions, kits and methods for making and using RNA compositions comprising in vitro synthesized ssRNA inducing a biological or biochemical effect in a mammalian cell or organism into which the RNA composition is repeatedly or continuously introduced. In certain embodiments, the invention provides compositions and methods for changing the state of differentiation or phenotype of a human or other vertebrate cell. For example, the present invention provides mRNA and methods for reprogramming cells that exhibit a first differentiated state or phenotype to cells that exhibit a second differentiated state or phenotype, such as to reprogram human somatic cells to pluripotent stem cells.
-26. (canceled)
A treated RNA composition comprising in vitro-synthesized ssRNA or mRNA, wherein less than 0.01% of the mass of RNA in said treated RNA composition is double-stranded RNA (dsRNA) of a size greater than about 40 basepairs in length;
A method comprising treating an RNA composition with a buffered aqueous solution containing a dsRNA-specific endoribonuclease III protein, magnesium cations at a concentration of about 1-4 mM, and a salt providing an ionic strength equivalent to at least about 50 mM potassium acetate or potassium glutamate, wherein the RNA composition comprises either mRNA or a ssRNA precursor to said mRNA prior to it being capped and/or polyadenylated so that the amount of dsRNA that is greater than about 40 basepairs in length following said treating is less than 0.01% of the mass of RNA in said treated RNA composition.
Dependencies are inferred from the claim text.