Rapid multiplexed analysis of released glycans by dual-stage microfluidic capture and parallel fluorescent tagging prior to lc analysis

A method of capturing glycans released from a protein or polypeptide, the method comprising:

• passing the protein or polypeptide through a polypeptide capture device comprising an affinity capture agent, wherein at least a portion of the protein or polypeptide passing through the polypeptide capture device is captured to the affinity capture agent;
• treating the captured protein or polypeptide with a glycanase such that one or more glycans are removed from the captured protein or polypeptide;
• and
• passing the released glycans into a glycan capture and labeling device, wherein the glycan capture and labeling device comprises one or more glycan affinity capture agents, wherein the one or more glycan affinity capture agents have an affinity for a specific structural feature of a glycan.