Method for detecting an analyte of interest in a sample
AssigneeRoche Diagnostics Operations, Inc.
InventorsBikram Keshari Agrawalla, Francesco Ansideri, Dieter Heindl, Lars Hillringhaus, Hannes Kuchelmeister, Giuseppe Prencipe, Sona Simonyiova
Life of this patent
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Abstract
The present invention relates to a method for determining at least one analyte of interest and the use thereof. The present invention further relates to a kit, a complex, a method to synthesize a complex, a monomer and the use thereof for detecting the analyte of interest in the sample.
Claims22
A method for detecting an analyte of interest in a sample comprising the steps of
- a) providing the sample comprising the analyte of interest;
- b) providing a complex comprising a linker, wherein the linker covalently binds a label compound and an analyte-specific binding agent, wherein the label compound is capable of producing a detectable signal;
- more preferably an electrochemiluminescence based signal,
- c) coupling the sample of step a) with the complex of step b);
- and
- d) detecting the analyte of interest by using the detectable signal of the label compound,
- wherein the complex is a compound of formula I:
- wherein A represents the label compound and B represents the analyte-specific binding agent or vice versa,
- X is selected from the group consisting of O, S, CH2, SO, SO2, 1,2,3-triazole, and amide,
- a is 1 to 5,
- b is 0 to 3, and
- n is an integer from 2 to 20.
(canceled)
A kit for performing detection of an analyte of interest in a sample, the kit comprising in separate containers
- a) a solid phase capable of immobilizing the analyte;
- and
- b) a compound of formula I:
- wherein A represents the label compound and B represents the analyte-specific binding agent or vice versa,
- is selected from the group consisting of O, S, CH2, SO, SO2 or 1,2,3-triazole, and amide,
- a is 1 to 5,
- b is 0 to 3, and
- n is an integer from 1 to 20 or from 2 to 20.
(canceled)
A complex of formula I,
- wherein A represents a label compound and B represents an analyte-specific binding agent or vice versa,
- X is selected from the group consisting of O, S, CH2, SO, SO2 or 1,2,3-triazole, and amide,
- a is 1 to 5,
- b is 0 to 3, and
- n is an integer from 1 to 20 or from 2 to 20.
A monomer used for a peptide-based synthesis comprising the following formula II
- wherein PG1, PG2, PG3, PG4, PG5, PG6 are each independently a protecting group,
- wherein X is selected from the group consisting of O, S, CH2, SO, SO2 or 1,2,3-triazole, and amide,
- wherein a is 1 to 5,
- wherein b is 0 to 3.
Dependencies are inferred from the claim text.