System and kit for rapid multimodal detection of anaerobic sulfate-reducing bacteria via integrative spectral comparison

A kit, comprising:

• one or more first reagents formulated to indicate via a first set of spectral parameters a level of hydrogen sulfide (H2S) in a fluid test sample;
• one or more second reagents formulated to indicate via a second set of spectral parameters, a level of enzymatic activity of one or more species of anaerobic sulfate-reducing bacteria (SRB) in the fluid test sample;
• and
• one or more oxygen scavengers combined with the one or more second reagents and formulated to, upon contact with the fluid test sample, provide an anaerobic environment for the one or more species of anaerobic sulfate-reducing bacteria.

A method for providing a kit for field use in rapid multimodal detection of anaerobic sulfate-reducing bacteria (SRB) in a fluid test sample via integrative spectral comparison, the method comprising:

• determining a first set of spectral parameters for one or more first reagents formulated to indicate, via the first set of spectral parameters, one or more predetermined levels of hydrogen sulfide (H2S) in a fluid test sample;
• determining a second set of spectral parameters for one or more second reagents formulated to indicate, via the second set of spectral parameters, one or more predetermined levels of enzymatic activity of one or more species of anaerobic sulfate-reducing bacteria in the fluid test sample;
• providing a first spectral reference standard comprising a set of spectral parameters representative of one or more predetermined levels of hydrogen sulfide (H2S) in a first spectral reference configured for performing a first comparison with a first spectral signal to be generated in response a reaction of the one or more first reagents with H2S upon contact with the fluid test sample;
• and
• providing a second spectral reference standard comprising a set of spectral parameters representative of or more predetermined levels of enzymatic activity of one or more species of anaerobic sulfate-reducing bacteria in a second spectral reference based on a second comparison with a second spectral signal generated in response to microbial interaction with a combination of the one or more second reagents and one or more oxygen scavengers that are formulated to, upon contact with the fluid test sample, maintain an anaerobic environment for the one or more species of anaerobic sulfate-reducing organisms.