Cell count and cell viability assessment using water proton nmr
AssigneesUniversity of Maryland, Baltimore, Government of the United States of America, AS Represented By the Secretary of Commerce
InventorsYihua Bruce YU, Marc B. TARABAN, Katharine T. BRIGGS, Zvi KELMAN, William Bradley O'DELL
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Abstract
A method of using the relaxation rate (R1 and/or R2) of solvent NMR signal to non-invasively estimate cell counts in cell cultures prepared under similar conditions as well as to use wNMR to measure cell viability. The methods can simply, rapidly, and non-invasively monitor the growth, health, and aging status of a cell culture, without any sample preparation or reagent (e.g., a dye) addition.
Claims20
A method of estimating a cell count in an aliquot comprising cells, said method comprising measuring the relaxation rate of solvent R in the aliquot comprising cells, and comparing the measured R of solvent to a calibration curve to estimate the cell count of the aliquot comprising cells,
- wherein the R is selected from the transverse relaxation rate of solvent R2 or the longitudinal relaxation rate of solvent R1.
A method of estimating a percent cell viability in an aliquot comprising cells, said method comprising measuring the relaxation rate of solvent R in the aliquot comprising cells, and comparing the measured R of solvent to a calibration curve to estimate the percent cell viability of the aliquot comprising cells,
- wherein the R is selected from the transverse relaxation rate of solvent R2 or the longitudinal relaxation rate of solvent R1.
Dependencies are inferred from the claim text.