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US 2025/0223643 A1Published application
Reagent kit and sequencing method using the same
AssigneeMGI Tech Co., Ltd.
InventorsMan JIA, Lan JIANG, Zhenhao LU, Xintong TANG, Min ZHENG, Feng ZHANG, Chongjun XU
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Abstract
Provided are a reagent kit and use thereof in sequencing. The reagent kit includes a first reagent and a second reagent. The first reagent is selected from a disulfide bond reducing agent. The second reagent is selected from a blocking reagent suited to blocking a disulfide bond.
Claims20
A kit, comprising:
- a first reagent selected from a disulfide-reducing agent;
- and
- a second reagent selected from a blocking reagent suitable for blocking a disulfide bond.
A sequencing method using a kit, the kit comprising:
- a first reagent selected from a disulfide-reducing agent;
- and
- a second reagent selected from a blocking reagent suitable for blocking a disulfide bond,
- the method comprising:
- performing a multiple displacement amplification treatment on a template strand on a chip, to obtain a complementary strand, wherein a DNA polymerase with strand displacement activity is used in the multiple displacement amplification treatment;
- loading the first reagent onto the chip, and removing the first reagent through elution;
- loading the second reagent onto the chip, incubating, and removing the second reagent through elution;
- and
- performing a sequencing reaction, wherein the sequencing reaction is performed on the template strand and the complementary strand, or wherein the sequencing reaction is performed on the complementary strand.
6 more elements
Dependencies are inferred from the claim text.