Novel regulatory element for enhancing rna stability or mrna translation, zcchc2 interacting therewith, and use thereof
AssigneesSeoul National University R&Db Foundation, Institute for Basic Science
InventorsVic Narry KIM, Jenny SEO, Soo-Jin JUNG
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Abstract
The present disclosure relates to a novel regulatory element for enhancing RNA stability or mRNA translation; ZCCHC2 interacting with the regulatory element; and uses thereof. Being capable of increasing the expression of a target protein, the novel regulatory element for enhancing RNA stability or mRNA translation; and ZCCHC2 interacting with regulatory element according to the present disclosure are applicable in various fields, depending on the uses of the target protein.
Claims19
A construct comprising:
- a gene encoding a target protein;
- and
- a regulatory element, wherein the regulatory element comprises:
- (i) the nucleotide sequence of a segment of the Aichi virus 1 gene (NCBI Reference Sequence:
- NC_001918.1), or an RNA nucleotide sequence thereof, wherein the segment comprises more than 110 and up to 250 consecutive nucleotides in the 5′ direction from the nucleotide at position 8251 of the Aichi virus 1 genome;
- (ii) a nucleotide sequence having at least 90% identity to the (i) nucleotide sequence;
- or
- (iii) a nucleotide sequence which is within a 3′UTR of a kobuvirus genus and has at least 50% homology to the (i) nucleotide sequence.
A method for enhancing RNA stability or mRNA translation, using a composition comprising ZCCHC2 interacting with a regulatory element;
- or a gene encoding ZCCHC2.
A method for enhancing RNA stability or mRNA translation, using a regulatory element, wherein the regulatory element comprises:
- (i) the nucleotide sequence of a segment of the Aichi virus 1 gene (NCBI Reference Sequence:
- NC_001918.1), or an RNA nucleotide sequence thereof, wherein the segment comprises more than 110 and up to 250 consecutive nucleotides in the 5′ direction from the nucleotide at position 8251 of the Aichi virus 1 genome;
- (ii) a nucleotide sequence having at least 90% identity to the (i) nucleotide sequence;
- or
- (iii) a nucleotide sequence which is within a 3′UTR of a kobuvirus genus and has at least 50% homology to the (i) nucleotide sequence.
Dependencies are inferred from the claim text.