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US 2025/0215401 A1Published application
Compositions and methods for recombinant aav production
AssigneeREGENXBIO Inc.
InventorsPing LIU, Ayda MAYER, David C. JAMES, Thilo H. POHLE, Yusuf B. JOHARI
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Abstract
Provided herein are recombinant polynucleotides encoding helper functions and helper plasmids suitable for use in the production of recombinant AAV particles. Also provided herein are methods for producing rAAV particles.
Claims119
An isolated recombinant polynucleotide comprising one or more of
- a) a nucleotide sequence encoding an adenovirus E2A DNA binding protein (DBP) operably linked to a first promoter and to a first polyA signal;
- b) a nucleotide sequence encoding an adenovirus E4 polypeptide operably linked to a second promoter and a second polyA signal;
- and
- c) a nucleotide sequence encoding an adenovirus VA RNA I,
- wherein the isolated recombinant polynucleotide does not comprise a nucleotide sequence encoding an adenovirus ITR sequence, L3 23K endoprotease, L5 pVI/fibre, and/or L4 pVIII/hexon-associated precursor.
2 more elements
Dependencies are inferred from the claim text.