Method for producing inner ear organoids using retinoic acid and inner ear organoids produced by the method
AssigneeAjou University Industry-Academic Cooperation Foundation
InventorsYun-Hoon Choung, Jeong Hun Jang, Young sun Kim, Jungho Ha
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Abstract
The present disclosure relates to a method for producing an inner ear organoid with improved differentiation efficiency of hair cells. The inventors of the present disclosure have confirmed that when retinoic acid is treated at the stage of formation of the lamina propria during the production of an inner ear organoid, the differentiation potential of hair cells is excellent. Therefore, it is expected that the inner ear organoid produced according to the production method of the present disclosure can be advantageously utilized in hearing loss disease modeling, drug screening and regenerative medicine.
Claims10
A composition for producing an inner ear organoid, comprising retinoic acid.
A method for producing an inner ear organoid with a promoted differentiation potential of hair cells, the method comprising:
- a) culturing embryonic stem cells or induced pluripotent stem cells to form a true endoderm;
- b) forming a non-neural ectoderm;
- c) forming an otic-epibranchial progenitor domain;
- d) forming an otic placode;
- and
- e) differentiating hair cells, supporting cells and neurons,
- wherein a composition comprising retinoic acid is treated in step d).
Dependencies are inferred from the claim text.