Composition and method for detection of biomolecules via aromatic labeling using heterocyclic compounds
AssigneesLife Technologies Corporation, Pierce Biotechnology, Inc.
InventorsThomas Diller, Premchendar Nandhikonda
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Abstract
Disclosed herein is a composition comprising a medium and a heterocyclic compound, for detecting a biomolecule in a sample. More specifically, the composition disclosed herein can be used to detect one or more biomolecules comprising one or more aromatic amino acid residues by producing an amino acid conjugate upon associating with the aromatic amino acid residue of the biomolecule. Also disclosed herein are methods of making and using the composition disclosed herein.
Claims31
A composition for detecting a biomolecule, comprising:
- a medium;
- and
- a heterocyclic compound according to Formula I, or an enantiomer, diastereomer, tautomer, salt, solvate, and/or isotopically substituted derivative thereof,
- wherein
- each of J, Q, T, X, Y, and Z independently is selected from O;
- P;
- B;
- N;
- S;
- Se;
- N(R″) or N+[R″], wherein R″ independently is selected from hydrogen, aliphatic, cycloaliphatic, cycloheteroaliphatic, or aryl;
- or BRc or C(Rc)n, wherein n is 1 or 2, and
- (i) each Rc of BRc or C(Rc)n independently is selected from hydrogen, halo, aliphatic, heteroaliphatic, ether, amino, carbonyl, carboxyl, ketone, aldehyde, isocyanate, cyano, oxime, nitro, nitrile, a salt thereof, or an anionic form thereof, or
- (ii) for C(RC)n, two Rc groups, together with the carbon atoms to which they are attached, form an aliphatic ring system, a heteroaliphatic ring system, or an aromatic ring system;
- provided that at least one of J, Q, T, X, Y, and Z is O, P, N, S, Se, N(R″), N+[R″], or BRc;
- and m is zero is or one;
- and
- wherein the heterocyclic compound is selected to associate with an aromatic amino acid residue of the biomolecule and wherein the heterocyclic compound produces a conjugate upon association with the aromatic amino acid residue of the biomolecule.
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A composition comprising:
- a buffer;
- a polyacrylamide derived from acrylamide and bisacrylamide;
- a biomolecule comprising an aromatic amino acid residue;
- and
- an associating means for producing an amino acid conjugate upon associating with the aromatic amino acid residue.
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Dependencies are inferred from the claim text.