Acoustic separation of particles for bioprocessing
AssigneeThe Charles Stark Draper Laboratory, Inc.
InventorsJason O. Fiering, Kenneth T. Kotz
Life of this patent
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Abstract
A method for separating particles in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic separation, capable of separating target particles from non-target particles in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic particle separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use.
Claims19
A kit for microfluidic particle separation comprising:
- at least one microfluidic separation channel configured to separate target particles from non-target particles in a biofluid, the separation channel comprising at least one inlet, a first outlet, and a second outlet, the separation channel connected to at least one acoustic transducer;
- a source of an additive fluidly connectable with the microfluidic separation channel, the additive capable of altering at least one of size of the target particles, size of the non-target particles, compressibility of the biofluid, compressibility of the target particles, compressibility of the non-target particles, aggregation potential of the target particles, and aggregation potential of the non-target particles;
- and
- instructions to provide the biofluid, pretreat the biofluid by introducing a predetermined volume of the additive into the biofluid, flow the pretreated biofluid into the at least one inlet of the microfluidic separation channel, and apply acoustic energy to the microfluidic separation channel.
A kit for microfluidic particle separation comprising:
- at least one microfluidic separation channel configured to separate target particles from non-target particles in a biofluid, the separation channel comprising at least one inlet, a first outlet, and a second outlet, the separation channel coupled with at least one acoustic transducer;
- a source of an additive fluidly connectable with the microfluidic separation channel, the additive capable of altering at least one attribute of at least one of the non-target particles and target particles;
- and
- instructions to provide the biofluid, pretreat the biofluid by introducing a predetermined volume of the additive into the biofluid, flow the pretreated biofluid into the at least one inlet of the microfluidic separation channel, and apply acoustic energy to the microfluidic separation channel.
Dependencies are inferred from the claim text.