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AssigneeIllumina, Inc.
InventorsXin Sheng, Jian-Sen Li, Aaron Aslanian, Melissa Carpenter, Thomas Richard Gros, Nassim Ataii, Thais Takei, Maria Annabelle Nulud Mesina-Gross
Estimating the term…
Some embodiments of the methods and compositions provided herein relate to blocked substrates in which non-specific binding of nucleic acids to the substrate is reduced. Some embodiments include use of carrier nucleic acids. More embodiments include the use of beads contacted with an oligonucleotide, such as an oligonucleotide containing one or more phosphorothioate bonds. Such substrates are useful in methods for obtaining long-read information from short reads of a target nucleic acid.
1-115. (canceled)
A method for reducing non-specific nucleic acid binding to a substrate, or normalizing a level of non-specific nucleic acid binding to the substrate, comprising:
A blocked bead composition comprising a magnetic bead in contact with an oligonucleotide, wherein the oligonucleotide comprises a backbone comprising a phosphorothioate bond, and wherein non-specific nucleic acid binding to the blocked bead is reduced compared to non-specific nucleic acid binding to a bead not in contact with the oligonucleotide, wherein the oligonucleotide comprises one or more of:
The method of 122, wherein the plurality of the transposomes is immobilized on the bead at a density such that:
Dependencies are inferred from the claim text.