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US 2025/0197908 A1Published application
Rna polymerase variants
AssigneeModernaTX, Inc.
InventorsAmy E. Rabideau, Athanasios Dousis, Kanchana Ravichandran, Elissa Hobert
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Abstract
The present disclosure provides, in some aspects, variant RNA polymerases, the use of which increases transcription efficiency while reducing the number of double-stranded RNA contaminates and run-on transcripts produced during an in vitro transcription reaction.
Claims16
-16. (canceled)
A T7 ribonucleic acid (RNA) polymerase variant comprising:
- an amino acid sequence having at least 99% identity to the amino acid sequence of SEQ ID NO:
- 1 modified to comprise an amino acid substitution at position G47 and an additional C-terminal amino acid,
- wherein the additional C-terminal amino acid comprises glycine (G).
-77. (canceled)
A co-transcriptional capping method for ribonucleic acid (RNA) synthesis, the method comprising:
- reacting a polynucleotide template with a T7 RNA polymerase variant, nucleoside triphosphates, and a cap analog, under in vitro transcription reaction conditions to produce RNA transcript,
- wherein the T7 RNA polymerase variant comprises an amino acid sequence having at least 99% identity to the amino acid sequence of SEQ ID NO:
- 1 modified to comprise an amino acid substitution at position G47 and an additional C-terminal amino acid,
- wherein the additional C-terminal amino acid comprises glycine (G).
1 more element
Dependencies are inferred from the claim text.