Legionaminic acid glycosyltransferases for chemoenzymatic synthesis of glycans and glycoconjugates
AssigneeThe Regents of the University of California
InventorsXi CHEN, Xiaohong YANG, Hai YU, Bijoyananda MISHRA
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Abstract
Provided herein are methods for preparing a glycan product containing legionaminic acid moieties and other nonulosonic acids. Also provided herein are legionaminic acid transferase fusion proteins and vaccine compositions containing glycan products prepared according to the described methods.
Claims35
A method for preparing a glycan product containing a nonulosonic acid moiety, the method comprising:
- (i) forming a reaction mixture comprising a legionaminic acid transferase (LegT), a donor comprising a nonulosonic acid moiety, and a glycan acceptor, and
- (ii) maintaining the reaction mixture under conditions for LegT-catalyzed transfer of the nonulosonic acid moiety from the donor to the glycan acceptor, thereby forming the glycan product containing the nonulosonic acid moiety.
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A legionaminic acid transferase fusion protein comprising a polypeptide having at least 80% sequence identity to SEQ ID NO:1, wherein the N-terminus of the polypeptide is fused to a maltose binding protein.
Dependencies are inferred from the claim text.