Recombinant virus-like particle capsid vaccines against adenoviruses and compositions, methods, and use thereof
AssigneesTechnoVax, Inc., The Government of the United States, as represented by the Secretary of the Army
InventorsJose M. GALARZA, Ke WEN, Robert KUSCHNER
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Abstract
The present application relates to adenovirus virus-like particles (AdVLPs) and related compositions, plasmids, and methods. The AdVLP can include a recombinant capsid that comprises major capsid adenovirus (AdV) proteins such as a hexon protein, a penton protein, and a fiber protein, and minor capsid/cement AdV proteins, such as a Illa protein, a VI protein, a VIII protein, and a IX protein. The minor capsid/cement AdV proteins structurally support the major capsid AdV proteins. The recombinant capsid of the AdVLP can further include a chaperone AdV protein, and an accessory scaffold AdV protein.
Claims30
An adenovirus virus-like particle (AdVLP) comprising:
- a recombinant capsid comprising:
- a) major capsid adenovirus (AdV) proteins including a hexon protein, a penton protein, and a fiber protein,
- b) minor capsid/cement AdV proteins, including a IIIa protein, a VI protein, a VIII protein, and a IX protein, wherein the minor capsid/cement AdV proteins structurally support the major capsid AdV proteins,
- c) a chaperone AdV protein L4-100k, and
- d) an accessory scaffold AdV protein L1-52/55k.
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An expression plasmid comprising genes encoding adenovirus proteins, wherein the expression plasmid is suitable for the assembly of adenovirus virus-like particles (AdVLPs) and wherein the expression plasmid comprises codon-optimized genes encoding:
- a) major capsid adenovirus (AdV) proteins including a hexon protein, a penton protein, and a fiber protein,
- b) minor capsid/cement AdV proteins, including a IIIa protein, a VI protein, a VIII protein, and a IX protein,
- c) a chaperone AdV protein L4-100k, and
- d) an accessory scaffold AdV protein L1-52/55k.
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A method of producing an adenovirus virus-like particle (AdVLP) comprising:
- introducing into a host cell at least one expression plasmid under conditions such that the host cell produces the AdVLP, wherein the at least one expression plasmid comprises codon-optimized genes encoding:
- a) major capsid adenovirus (AdV) proteins including a hexon protein, a penton protein, and a fiber protein,
- b) minor capsid/cement AdV proteins, including a IIIa protein, a VI protein, a VIII protein, and a IX protein,
- c) a chaperone AdV protein L4-100k, and
- d) an accessory scaffold AdV protein L1-52/55k.
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Dependencies are inferred from the claim text.