Compositions and methods for epigenome editing to enhance t cell therapy
AssigneeDuke University
InventorsCharles A. Gersbach, Sean McCutcheon
Life of this patent
Estimating the term…
Abstract
Disclosed herein are compositions and methods for modulating T cells. For example, the compositions and methods may be used to increase memory T cells. The compositions and method may be used in combination with Adoptive T Cell Therapy (ACT) to enhance the ACT.
Claims53
A composition for modulating T cells, the composition comprising a modulator of a gene selected from BATF3, BATF, EOMES, BHLHE40, CREM, NFE2L1, NR1D1, POU2F1, FOXD2, GABPA, RREB1, JUN, ZFP1, IRF2, NFATC3, NR4A1, DNMT1, FOXO1, MYB, TCF7L1, BACH2, HIC1, KLF2, and F11.
A DNA targeting composition comprising:
- a Cas9 protein or a fusion protein, wherein the fusion protein comprises two heterologous polypeptide domains, wherein the first polypeptide domain comprises a Cas9 protein and the second polypeptide domain has an activity selected from transcription activation activity, transcription repression activity, nuclease activity, transcription release factor activity, histone modification activity, nucleic acid association activity, methylase activity, and demethylase activity;
- and
- at least one guide RNA (gRNA) that targets the Cas9 protein to a target gene or a regulatory element thereof, wherein the target gene is selected from BATF3, BATF, EOMES, BHLHE40, CREM, NFE2L1, NR1D1, POU2F1, FOXD2, GABPA, RREB1, JUN, ZFP1, IRF2, NFATC3, NR4A1, DNMT1, FOXO1, MYB, TCF7L1, BACH2, HIC1, KLF2, and FLI1.
A composition for increasing T cells, the composition comprising an activator of a gene selected from BATF3, EOMES, NR1D1, and JUN.
A composition for increasing T cells, the composition comprising an inhibitor of a gene selected from BATF, DNMT1, FOXO1, MYB, and BACH2.
A composition for increasing T cells, the composition comprising an activator of the BATF3 gene.
The composition of any one of claims 1-43, further comprising at least one cancer therapy.
Dependencies are inferred from the claim text.