Synthetic virus and provision thereof

Method for in vitro amplification of a virus or bacteriophage nucleic acid, in particular a bacteriophage genome, comprising the steps of

• (a) providing a virus or bacteriophage nucleic acid template to be amplified,
• (b) amplification of the virus or bacteriophage nucleic acid template using polymerase chain reaction to amplify and provide different nucleic acid segments, wherein the primers used for at least one 5′-end of the virus or bacteriophage nucleic acid template optionally introduce nucleic acid exonuclease degradation blocking modifications, in particular phosphorothioate bonds, between the first nucleotides at the at least one 5′-end of the corresponding nucleic acid segments,
• (c) recession by an 5′-3′-directional exonuclease, in particular T5 exonuclease, to provide sticky end nucleic acid segments,
• (d) annealing of the sticky nucleic acid segments and
• (e) ligating of the annealed nucleic segments to provide amplified linear bacteriophage nucleic acids.