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US 2025/0197807 A1Published application
Methods and compositions for producing granulosa-like cells
AssigneePresident and Fellows of Harvard College
InventorsMerrick Pierson Smela, Christian Kramme, Pranam Chatterjee, George M. Church
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Abstract
Provided herein are methods and compositions for differentiating induced pluripotent stem cells into granulosa-like cells by overexpressing transcription factors such as NR5A1 and a RUNX family protein (e.g., RUNX1 and/or RUNX2).
Claims58
A pluripotent stem cell (PSC) comprising:
- an engineered polynucleotide comprising an open reading frame encoding a protein selected from NR5A1 and a RUNX family protein.
A pluripotent stem cell (PSC) comprising:
- a protein selected from NR5A1 and a RUNX family protein, wherein the protein is overexpressed.
A method, comprising:
- culturing, in culture media, a population of pluripotent stem cells (PSCs) to produce an expanded population of PSCs;
- and expressing in PSCs of the expanded population a protein selected from NR5A1 and a RUNX family protein to produce granulosa-like cells.
A method comprising:
- (a) delivering to pluripotent stem cells (PSCs) an engineered polynucleotide comprising an inducible promoter operably linked to an open reading frame encoding a protein selected from NR5A1 and a RUNX family protein;
- (b) culturing the PSCs in feeder-free, serum-free culture media to produce an expanded population of PSCs;
- and
- (c) culturing PSCs of the expanded population in a series of induction media comprising an inducing agent to produce AMHR2+, CD82+, FOXL2+, and/or EPCAM− granulosa-like cells.
1 more element
Dependencies are inferred from the claim text.