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US 2025/0197794 A1Published application
Membrane rupture compositions and methods of making and using same
AssigneeAvantor Performance Materials, Inc.
InventorsArvind Srivastava, Nandkumar Deorkar, Lori Fortin, Jungmin Oh, Courtney O'Dell, Pranav S. Vengsarkar
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Abstract
The present invention provides a membrane rupture solution comprising: one or more purified non-ionic detergents, wherein at least one of the detergents has a surface activity property that is suitable for viral vector/protein stabilization against shear stress, and, optionally, a scavenger.
Claims22
A membrane rupture solution comprising:
- one or more purified non-ionic detergents, wherein the non-ionic detergent is selected from the group consisting of ethoxylated alcohol, sorbiton ethoxylate, triblock copolymer and combinations thereof, wherein the detergent is in a concentration range from about 0.01% (w/v) to about 2.0% (w/v);
- and
- a scavenger, in a concentration in a range of 0% to about 2.0%.
A method of purifying TDA9 to reduce product-related impurities, comprising:
- (1) heating raw material to 50-180° C. while mixing with an inert gas or applying vacuum, wherein the raw material comprises impurities and TDA9;
- (2) cooling the material of step (1) to 15-40° C. while mixing with inert gas;
- (3) adding 10-50 volume percent of water to the material of step (2) to form a homologized solution;
- (4) heating the solution of step (3) to 40-120° C. to complete hydrolysis of volatile impurities;
- and
- (5) filtering the solution of step (4) with a 0.2-1.0 μm filter,
- wherein the TDA9 is purified.
4 more elements
Dependencies are inferred from the claim text.