Method for producing polyoxyethylene derivative, method for quantitating polyoxyethylene compound, and labeling reagent for analysis use
AssigneeNof Corporation
InventorsKen HAMURA, Masaki KAMIYA
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Abstract
A method for producing a high molecular weight polyoxyethylene derivative having a derivatized hydroxyl group is disclosed. The method includes labeling an unreacted high molecular weight polyoxyethylene compound having a hydroxy group using a labeling reagent in a deuterated solvent, and quantitating a labeled solution by a pre-saturation method and a proton nuclear magnetic resonance spectroscopy using inverse gated decoupling. The method allows a prompt quantitation of unreacted high molecular weight polyoxyethylene compound having a hydroxy group with a high accuracy.
Claims10
A method for producing a high molecular weight polyoxyethylene derivative having a derivatized hydroxy group by derivatizing a hydroxy group in a high molecular weight polyoxyethylene compound having the hydroxy group, the method comprising:
- labeling the high molecular weight polyoxyethylene compound having the hydroxy group, which is unreacted, using a labeling reagent in a deuterated solvent;
- and
- quantitating a labeled solution by a pre-saturation method and a proton nuclear magnetic resonance spectroscopy using inverse gated decoupling.
A labeling reagent for analysis use for a high molecular weight polyoxyethylene compound having a hydroxy group, which is represented by a formula (3):
- wherein, in the formula (3), R represents an organic group having an electron-withdrawing group.
A method for quantitating a high molecular weight polyoxyethylene compound having a hydroxy group contained in a high molecular weight polyoxyethylene derivative, the method comprising:
- labeling the high molecular weight polyoxyethylene compound having a hydroxy group using a labeling reagent in a deuterated solvent;
- and
- quantitating a labeled solution by a pre-saturation method and a proton nuclear magnetic resonance spectroscopy using inverse gated decoupling.
Dependencies are inferred from the claim text.