Processes for making and using a cellular fibronectin composition
AssigneeCombangio, Inc.
InventorsSpencer Alford, Audrey Smith
Life of this patent
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Abstract
The present application provides methods and processes for making and using a fibronectin composition, as well as methods for treating ocular conditions and/or disorders with the cellular fibronectin composition described herein.
Claims23
A composition comprising fibronectin (FN), wherein the FN is mesenchymal stem cell (MSC)-derived FN, wherein the MSCs are derived from bone marrow, wherein the FN is soluble, and wherein the composition does not comprise one or more components selected from the group consisting of:
- xenobiotic components;
- Phenol red;
- peptides and biomolecules<3 kDa;
- antibiotics;
- protein aggregates;
- cells;
- cell debris;
- hormones;
- and L-glutamine.
-23. (canceled)
(canceled)
-30. (canceled)
A composition comprising mesenchymal stem cell (MSC)-derived FN, wherein the composition is made by:
- (a) culturing mesenchymal stem cells (MSCs) in a cell culture medium, thereby generating conditioned medium that comprises factors comprising MSC-derived FN secreted by the MSCs;
- (b) harvesting the conditioned medium thereby producing harvested conditioned medium;
- and
- (c) filtering harvested conditioned medium to produce processed conditioned medium;
- wherein the MSCs are bone marrow-derived.
Dependencies are inferred from the claim text.