Methods for the reduction of methane production in ruminants

A method for reducing methane emissions from a ruminant, the method comprising administering to the ruminant bromoform produced by a composition comprising:

• bromide, and
• a vanadium-dependent haloperoxidase (VHPO) comprising SEQ ID NO:
• 2 or a variant protein thereof, wherein the variant protein comprises SEQ ID NO:
• 2 with a conservative amino substitution for isoleucine at position 3, tyrosine at position 69, serine at position 75, asparagine at position 81, alanine at position 82, threonine at position 111, threonine at position 120, alanine at position 138, isoleucine at position 141, lysine at position 147, threonine at position 163, lysine at position 215, isoleucine at position 261, phenylalanine at position 288, tyrosine at position 356, leucine at position 447, lysine at position 538, arginine at position 544, leucine at position 575, glutamine at position 581, glutamine at position 594, alanine at position 598, isoleucine at position 605, or a combination thereof;
• thereby reducing methane emissions in the ruminant relative to a ruminant not administered bromoform produced by the composition.