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US 2025/0180508 A1Published application
Quantitative lipidomic analysis, methods and uses thereof
AssigneeMcMaster University
InventorsPhilip Britz-McKibbin, Ritchie Ly
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Abstract
This disclosure relates to the field of lipidomics, and in particular, to a novel derivatization strategy for quantitative lipid methylation and uses thereof. Further, this disclosure relates to use of phospholipid biomarkers for assessing and monitoring Omega-3 Index (O3I), including methods and uses thereof.
Claims21
A method of generating a lipid profile using mass spectrometry (MS), the method comprising:
- a) extracting a lipid fraction from a sample obtained from a subject;
- b) protecting the amine of one or more lipids in the lipid fraction using an amine protecting reagent to provide one or more protected lipids;
- c) methylating a phosphate ester to generate a phosphate methyl ester of the one or more protected lipids using 3-methyl-1-p-tolyltriazene (MTT) to generate one or more methylated lipids;
- d) optionally back extracting the one or more methylated lipids;
- e) optionally separating the one or more methylated lipids;
- f) introducing the one or more methylated lipids to a mass spectrometer under positive-ion mode;
- and
- g) acquiring a mass spectrum chart of the one or more methylated lipids to generate the lipid profile of the sample.
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A method of assessing omega-3 index (O3I) status in a subject, the method comprising:
- a) obtaining a first sample from the subject at a first time point;
- b) measuring the level of one or more omega-3 containing phospholipid biomarkers in the first sample;
- and
- c) comparing the level of the one or more omega-3 containing phospholipid biomarkers in the first sample to a control level or value of the one or more omega-3 containing phospholipid biomarkers of known O3I status,
- wherein similarity of level of the phospholipid biomarkers in the sample with the control indicates that the sample is from a subject with the known O3I status.
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Dependencies are inferred from the claim text.