Method of producing cannabinoids
OwnerJamison Parker HUDDLESTON, Andreas SCHIRMER +2inventors
InventorsJamison Parker HUDDLESTON, Andreas SCHIRMER, Trevor Nelson PURDY, Benjamin Matthew GRIFFIN
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Abstract
The present disclosure provides an in vitro method for producing a cannabinoid, the method comprising reacting a N prenylated aromatic compound with a flavin-dependent oxidase in a reaction mixture to form the cannabinoid, wherein the flavin-dependent oxidase is not derived from C. sativa.
Claims43
An in vitro method for producing a cannabinoid. the method comprising reacting a prenylated aromatic compound with a flavin-dependent oxidase in a reaction mixture to form the cannabinoid, wherein the flavin-dependent oxidase is not derived from C. sativa.
An in vitro method for producing a cannabinoid. the method comprising reacting a prenylated aromatic compound with a flavin-dependent oxidase in a reaction mixture to form the cannabinoid, and wherein the flavin-dependent oxidase does not comprise a disulfide bond.
‘An in vitro method for producing a cannabinoid, the method comprising reacting a prenylated aromatic compound with a flavin-dependent oxidase in a reaction mixture, wherein the flavin-dependent oxidase is a prokaryote protein or fungal protein, or derivative thereof.
An in vitro method for producing a cannabinoid, the method comprising reacting a prenylated aromatic compound with a flavin-dependent oxidase in a reaction mixture, and wherein the flavin-dependent oxidase comprises at least 70% sequence identity to any of SEQ ID NOs:1-6 or any of SEQ ID NOs:15-37.
An in vitro method for producing a cannabinoid, the method comprising:
- a. culturing a prokaryote cell or fungal cell comprising a flavin-dependent oxidase in a fermenter;
- b. lysing the prokaryote cell or fungal cell to form a lysate comprising the flavin-dependent oxidase;
- c. adding a prenylated aromatic compound to the flavin-dependent lysate in vitro, whereby the prenylated aromatic compound can interact with the flavin-dependent oxidase to form the cannabinoid;
- and
- d. recovering the cannabinoid.
An in vitro method for producing a cannabinoid. the method comprising:
- a. culturing a prokaryote cell or fungal cell comprising a flavin-dependent oxidase in a fermenter, wherein the flavin-dependent oxidase does not comprise a disulfide bond;
- b. lysing the prokaryote cell or fungal cell to form a lysate comprising the flavin-dependent oxidase;
- c. adding the lysate to a chromatography apparatus comprising a solid support, whereby the flavin-dependent oxidase is immobilized onto the solid support;
- d. adding a prenylated aromatic compound to the chromatography apparatus, whereby the prenylated aromatic compound can interact with the flavin-dependent oxidase to form the cannabinoid;
- and
- e. recovering the cannabinoid.
A system for producing cannabinoid products, comprising:
- a. a fermenter holding a cell culture medium comprising prokaryote cells or fungal cells producing a flavin-dependent oxidase wherein the flavin-dependent oxidase does not comprise a disulfide bond;
- b. a means for lysing the prokaryote cells or fungal cells to form a lysate comprising the flavin-dependent oxidase;
- c. an apparatus containing a reaction mixture configured to interact the immobilized flavin-dependent oxidase with a prenylated aromatic compound to form a cannabinoid which is produced by the flavin-dependent oxidase.
A system for producing cannabinoid products comprising:
- a. a fermenter holding a cell culture medium comprising prokaryote cells or fungal cells producing a flavin-dependent oxidase wherein the flavin-dependent oxidase does not comprise a disulfide bond;
- b. a means for lysing the prokaryote cells or fungal cells to form a lysate comprising the flavin-dependent oxidase:
- c. an apparatus containing a reaction mixture configured to immobilize the flavin-dependent oxidase onto a solid support, and then interact the immobilized flavin-dependent oxidase with a prenylated aromatic compound to form a cannabinoid which is produced by the flavin-dependent oxidase.
A biphasic composition comprising:
- a. a prenylated aromatic compound in a first phase, wherein the first phase comprises an organic solvent;
- b. a flavin-dependent oxidase in a second phase, wherein the second phase comprises an aqueous solvent, and wherein the flavin-dependent oxidase does not comprise a disulfide bond.
A biphasic composition comprising:
- a. a prenylated aromatic compound in a first phase, wherein the first phase comprises an organic solvent;
- b. a flavin-dependent oxidase immobilized on a solid support in a second phase, wherein the second phase comprises an aqueous solvent, and wherein the flavin-dependent oxidase does not comprise a disulfide bond.
Dependencies are inferred from the claim text.