Cell growth supplements using dairy-based materials
AssigneeTerasaki Institute for Biomedical Innovation
InventorsSamad Ahadian, Wei Huang, Alireza Khademhosseini
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Abstract
The present disclosure relates to cell growth supplements using dairy-based materials for cell culture and other applications. Some embodiments are directed to sequentially culturing cells in a culture medium comprising factors to increase the relative size and/or density of the cells in the culture, e.g., whey protein. Other aspects of the disclosure relate to obtaining a milk product to produce the dairy-based material, e.g., milking a nursing mammal, milk-producing organoids, bacterial expression systems, etc. In some embodiments, the disclosure relates to techniques for isolating a whey protein from the milk product.
Claims107
A method, comprising:
- exposing a plurality of cells to cell culture media comprising whey protein;
- and
- growing the plurality of cells in the cell culture media until the cells increase in size by an average of least 450%, relative to the size of the cells prior to exposing the cells to the cell culture media.
The method of any one of claims 1-22, wherein the plurality of cells comprises goat cells. 24 The method of any one of claims 1-23, wherein the plurality of cells comprises bison cells.
A method, comprising:
- exposing a plurality of cells to a first cell culture media and growing the cells in the first culture media for at least 24 hours;
- exposing the plurality of cells to a second cell culture media comprising a whey protein;
- and
- growing the plurality of cells in the second cell culture media until the cells increase in size by an average of least 450%, relative to the size of the cells prior to the second cell culture media.
A method, comprising:
- obtaining a milk product from a milk-producing mammal;
- extracting a whey protein from the milk product;
- forming cell culture media comprising the whey protein;
- exposing a plurality of cells to the cell culture media;
- and
- growing the plurality of cells in the cell culture media until the cells increase in size by an average of least 450%, relative to the size of the cells prior to exposing the cells to the cell culture media.
A method, comprising:
- obtaining a milk product from a milk-producing organoid, wherein the organoid comprises a plurality of cultivated mammary epithelial cells;
- extracting a whey protein from the milk product;
- forming cell culture media comprising the whey protein;
- and
- exposing a plurality of cells to the cell culture media.
A method, comprising:
- obtaining a whey protein from a fermenting bacterial culture;
- forming a cell culture media comprising the whey protein;
- and
- exposing a plurality of cells to the cell culture media.
Dependencies are inferred from the claim text.