Automated Isolation of Viable Mitochondria

An automated isolation device for isolating viable mitochondria from cells cultured in a bio-incubator, the automated isolation device comprising:

• a processor configured to control operation of the automated isolation device;
• one or more bio-incubators, wherein the bio-incubators are configured to culture cells in preparation for mitochondria isolation;
• a pump configured to transfer the cultured cells from the bio-incubator to an incubation station;
• wherein the incubation station is configured to incubate the cultured cells in the incubation station in a sterile solution;
• a first transfer system including a first automated pump, the first transfer system configured to, after the incubation of the cells, transfer the sterile solution containing the cultured cells from the incubation station to a filtration station;
• the filtration station comprising a series of filters;
• a second transfer system including a second automated pump, the second transfer system configured to transfer filtered solution from the filtration station to a centrifugation station, the filtered solution comprising mitochondria;
• and
• the centrifugation station for isolation of the mitochondria from the filtered solution.