Sequence of activated protein c

A polypeptide or a partial polypeptide thereof,

• comprising an amino acid sequence represented by
• formula:
• A1—A2—A3 (I)
• wherein A1 is an amino acid sequence comprising an amino acid sequence of a light chain of protein C or a homologue thereof, A2 is an amino acid sequence constituting a self-cleaving site, a A3 is an amino acid sequence comprising an amino acid sequence of a heavy chain of protein C or a homologue thereof, and the C-terminal amino acid of A1 and the N-terminal amino acid of A3 are Arg at position 211 and Leu at position 212 of human protein C, respectively,
• wherein a dimeric protein consisting of fragments on the N-terminal side and C-terminal side of the cleaving site of A2, or a partial protein thereof, has protein C activity,
• wherein the polypeptide satisfies the following conditions:
• (1) an amino acid sequence (formula:
• A1—A3 (II)) in which A1 (N-terminal side) and A3 (C-terminal side) are linked comprises the amino acid sequence of SEQ ID NO:
• 2,
• (2) an amino acid sequence in which A1 (N-terminal side) and A3 (C-terminal side) are linked comprises an amino acid sequence in which 1 to 45 amino acids have been deleted, substituted, inserted, or added in the amino acid sequence represented by SEQ ID NO:
• 2, or
• (3) an amino acid sequence in which A1 (N-terminal side) and A3 (C-terminal side) are linked comprises an amino acid sequence having 90% or more identity to the amino acid sequence represented by SEQ ID NO:
• 2, and
• A2 is selected from the group consisting of RKRRKR (SEQ ID NO:
• 3), KRRKR (SEQ ID NO:
• 4), RKRRKRRKR (SEQ ID NO:
• 8), and RKRRKRRKRRKR (SEQ ID NO:
• 9).