Loading patent…
AssigneeCarryGenes Bioengineering, LLC
InventorsEdward L. PERKINS, Amy L. GREENE, Dominique BROCCOLI, Kara Pascarelli MANNE, Ola WINQVIST, John ANDERSSON, Marton KESZEI, Katarina LYBERG, Maria EKOFF, Julia REMNESTÅL
Estimating the term…
Aspects of the present invention relate to synthetic chromosomes that may be incorporated into leukocytes, wherein the synthetic chromosomes comprise nucleic acid sequences encoding multiple Chimeric Antigen Receptors (CARs). Such manipulated leukocytes can be used in medicine, notably in the treatment of a cancer such as in the treatment of cancer having solid tumors. The leukocytes may be lymphocytes, including tumor-infiltrating lymphocytes. T cells. NK cells or B cells. In a preferred aspect, the leucocytes are syngeneic and T cells.
A synthetic chromosome comprising two or more nucleic acid sequences encoding two or more chimeric antigen receptors (CARs), wherein the two or more CARs are different.
A synthetic chromosome according to any one of the preceding claims, wherein the chromosome comprises 5 or more such as 7 or more, 9 or more, 10 or more, 15 or more, 20 or more, or 25 or more nucleic acid sequences encoding CARs.
A synthetic chromosome according to any one of the preceding claims, wherein the chromosome comprises at the most 80 such as at the most 70, at the most 60, at the most 50, at the most 40 or at the most 30 nucleic acid sequences encoding CARs.
A synthetic chromosome according to any one of the preceding claims comprising one or more promoters independently controlling expression of one or more chemokine receptors.
A synthetic chromosome according to any one of the preceding claims, wherein one or more promoters are inducible.
A synthetic chromosome according to any one of the preceding claims comprising one or more insulators.
A synthetic chromosome according to any one of the preceding claims for use in cellular therapy.
A synthetic chromosome for use in enhancing a therapeutic response in or in the vicinity of a target tissue by providing CARs expressed by cells carrying the chromosome.
A cell comprising a synthetic chromosome as defined in any one of the preceding claims.
A cell according to any of the preceding claims for medical use, veterinary use, or diagnostic use.
Dependencies are inferred from the claim text.