Novel therapeutic comprising a secretome from mesenchymal stem cells of umbilicord tissue or wharton's jelly
AssigneeCelligenics Pte. Ltd.
InventorsAimin Yeo, Pamela Mok, Sixun Chen, Yi Bing Aw
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Abstract
The invention concerns a novel therapeutic comprising a secretome obtained from mesenchymal stem cells of umbilical cord tissue or of wharton's jelly tissue and a formulation or pharmaceutical composition including same; ideally, but not exclusively, for use in treating a wound; a method for making said therapeutic; and a method of treating a wound using said therapeutic.
Claims18
A therapeutic comprising a secretome obtained from mesenchymal stem cells of umbilical cord tissue or mesenchymal stem cells of wharton's jelly tissue wherein the secretome comprises or consists of the following components:
- Granulocyte colony-stimulating factor (G-CSF);
- Growth-regulated protein alpha (GRO-a);
- hepatocyte growth factor (HGF);
- Interleukin-6 (IL-6);
- Leukemia inhibitory factor (LIF);
- monocyte chemoattractant protein 3 (MCP-3);
- stem cell growth factor beta (SCGF-b);
- Tissue inhibitor of metalloproteinase 1(TIMP-1);
- Matrix metalloproteinase 3 (MMP-3);
- Angiopoietin-like Protein 4 (ANGPTL4) Tumour necrosis factor receptor RI (TNF-RI) and CD63+ exosomes.
A method for making a therapeutic comprising a secretome obtained from mesenchymal stem cells of umbilical cord tissue or mesenchymal stem cells of wharton's jelly tissue wherein the method comprises:
- i) culturing mesenchymal stem cells of the umbilical cord or mesenchymal stem cells of wharton's jelly to confluence;
- ii) removing growth medium and washing the cell layer with an isotonic salt solution;
- iii) incubating the cells with a growth factor free medium;
- then
- iv) incubating the cells with a growth factor medium;
- and
- v) collecting the cell secretome from said cells.
Dependencies are inferred from the claim text.