System and method for protein corona sensor array for early detection of diseases
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsOmid Farokhzad, Morteza Mahmoudi, Claudia Corbo
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Abstract
The present disclosure provides a system comprising a communication interface and computer for assigning a label to the biomolecule fingerprint, wherein the label corresponds to a biological state. The present disclosure also provides a sensor arrays for detecting biomolecules and methods of use. In some embodiments, the sensor arrays are capable of determining a disease state in a subject.
Claims138
A sensor array comprising a plurality of sensor elements,
- wherein the plurality of sensor elements differ from each other in at least one physiocochemical property,
- wherein the plurality of sensor elements comprises at least two sensor elements;
- and
- wherein each sensor element is able to bind a plurality of biomolecules in a sample to produce a biomolecule corona signature, wherein each sensor elements has a distinct biomolecule corona signature from the other.
The sensor array of any one of the preceding claims, wherein the biomolecule corona signature comprises at least one protein, at least one polypeptide, at least one lipid, at least one metabolome, at least one oligonucleotide or combination thereof.
The sensor array of any one of the preceding claims, wherein the first sensor element produces a first biomolecule corona and the second sensor element produces a second biomolecule corona when the sensor array is contacted with the sample.
The sensor array of any one of the preceding claims, wherein the at least one physiocochemical property is selected from the group consisting of composition, size, surface charge, hydrophobicity, hydrophilicity, surface functionality, surface topography, and shape.
The sensor array of any one of the preceding claims, wherein the sensor array further comprises a substrate.
The sensor array of any one of the preceding claims, wherein the plurality of sensor elements are a plurality of particles.
The sensor array of any one of the preceding claims, wherein the plurality of sensor elements are a plurality of particles immobilized on a substrate.
The sensor array of any one of the preceding claims, wherein the sensor elements are organic particles, non-organic particles or combinations thereof.
The sensor array of any one of the preceding claims, wherein the sensor elements are particles and the particles are selected from the group consisting of nanoparticles, microparticles, micelles, liposomes, iron oxide, graphene, silica, protein-based particles, polystyrene, silver, and gold particles, quantum dots, and combination therefore.
The sensor array of any one of the preceding claims, wherein the sensor array comprises at least 2 to at least 100 sensor elements.
A method of distinguishing states of a complex biological sample of a subject using a plurality of particles having surfaces with different physicochemical properties, wherein the method comprises:
- exposing the complex biological sample to the plurality of particles to permit binding of proteins of the complex biological sample to the plurality of particles, wherein a pattern of binding of proteins amongst the plurality of particles differs based on the physicochemical properties of the surfaces of the particles;
- defining a biomolecule fingerprint representative of proteins that bind to the plurality of particles;
- and
- associating the biomolecule fingerprint with a biological state of the subject.
A sensor array comprising a plurality of particles having surfaces with different physicochemical properties, wherein proteins of a complex biological sample bind to the plurality of particles upon exposure of the complex biological sample to the plurality of particles, wherein a pattern of binding of the proteins amongst the plurality of particles depends on the physicochemical property of a surface of the particle.
A sensor array comprising a plurality of liposomes,
- wherein the plurality of liposomes differ in at least one protein-binding property defined by a lipid-based surface of each liposome;
- wherein the lipid-based surface of each liposome contacts a subset of proteins of a sample at a lipid-protein interface, thereby binding the subset of proteins to produce a pattern of protein binding;
- wherein the pattern of protein binding of a first liposome is different than the pattern of protein binding of a second liposome differing from the first liposome in said at least one protein-binding property.
A method of identifying a biological state of a subject using a plurality of liposomes differing in at least one protein-binding property defined by a lipid-based surface of each liposome, wherein the method comprises:
- exposing the sample to the plurality of liposomes to permit binding of proteins of the sample to the plurality of liposomes, wherein a pattern of binding of the proteins differs amongst liposomes with different protein-binding properties;
- separating the proteins from the liposomes;
- defining a biomolecule fingerprint of the proteins separated from the liposomes;
- and
- associating the biomolecule fingerprint with a state of the complex biological sample of the subject.
Dependencies are inferred from the claim text.