Image acquisition device and acquisition method for detecting target material signal

An image acquisition device for detecting a target material signal, comprising:

• (i) a camera for detecting a fluorescence signal and an optical signal;
• (ii) a sample unit that is installed at a certain interval from the camera;
• (iii) a fluorescence unit that supplies excitation light from the camera toward the sample unit;
• and
• (iv) an optical section that is installed at a certain distance from the sample unit in an opposite direction to the camera and supplies visible light toward the camera,
• wherein the fluorescence unit includes:
• (1) light sources that are disposed outside the camera at regular intervals in a circular shape;
• (2) a shielding guide that is positioned above the light source and transmits only vertical direction light of light emitted from the light source;
• and
• (3) a prism that refracts the light passing through the shielding guide toward a sample.

An image analysis method using an image acquisition device for detecting a target material signal, the image acquisition device including:

• (i) a camera for detecting a fluorescence signal and an optical signal, (ii) a sample unit that is installed at a certain interval from the camera, (iii) a fluorescence unit that supplies excitation light from the camera toward the sample unit, and (iv) an optical section that is installed at a certain distance from the sample unit in an opposite direction to the camera and supplies visible light toward the camera, the image analysis method comprising:
• {circle around (1)} injecting magnetic particles bound to a target material into each well of a multi-well plate;
• {circle around (2)} fixing the multi-well plate to a sample unit;
• {circle around (3)} applying power to an upper optical unit to acquire an optical image;
• and
• {circle around (4)} applying power to a lower fluorescence unit to acquire a fluorescence image.