Flavin-dependent oxidases having cannabinoid synthase activity

Abstract

The disclosure relates to flavin-dependent oxidases having cannabinoid synthase activity. The flavindependent oxidase comprises: (i) a first amino acid sequence comprising a His residue, wherein an FAD cofactor is covalently attached to the His residue; and (ii) a second amino acid sequence comprising a peptide motif of Formula (I): X1-Gly-X2-Cys-X3—X4—X5—X6—X7—X8-Gly-X9—X10—X11-Gly-Gly-Gly-X12-Gly, wherein each X is any amino acid; and wherein the FAD cofactor is covalently attached to the Cys residue, wherein the flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid, and wherein the flavin-dependent oxidase is a bacterial protein or a fungal protein.

Dependencies are inferred from the claim text.