Nucleic acid compositions for delivering exogenous polynucleotides and methods of use
AssigneeVyro Bio Inc.
InventorsCarolini Kaid DAVILA, Mauro Ferreira DE AZEVEDO
Life of this patent
Estimating the term…
Abstract
Disclosed herein are nucleic acid compositions for delivering exogenous polynucleotides, and methods of use.
Claims24
A method of delivering a polynucleotide to a target cell in a subject, the method comprising administering to the subject a zika virus or a zika-virus-like particle comprising the polynucleotide, wherein the polynucleotide is not a zika virus polynucleotide, and the target cell is a cell of the central nervous system.
A method of delivering a polynucleotide to a subject, the method comprising administering to the subject a zika virus or a zika-virus-like particle comprising the polynucleotide, wherein the polynucleotide is not a zika virus polynucleotide, and the zika virus or zika-virus-like particle does not comprise a replicating zika virus.
A method of delivering a polynucleotide to a subject, the method comprising administering to the subject a zika virus or a zika-virus-like particle comprising the polynucleotide, wherein the polynucleotide is not a zika virus polynucleotide, and the polynucleotide has a length up to about 10000 nucleotides.
A zika virus or zika-virus-like particle comprising a polynucleotide exogenous to zika virus having a length of about 6,000 nucleotides to about 10,000 nucleotides, optionally wherein the polynucleotide does not encode a full-length viral structural protein or a full-length viral nonstructural protein.
A nucleic acid composition comprising (i) a 5′ untranslated region (5′ UTR) of a first zika virus, (ii) a 3′ UTR of a second zika virus, wherein the first zika virus is optionally the same as the second zika virus, and (iii) a polynucleotide exogenous to the first zika virus and the second zika virus.
Dependencies are inferred from the claim text.