Method for the fermentative production of guanidinoacetic acid

2:

• The microorganism of claim 1, wherein the increased activity of the enzyme having the function of a carbamoylphosphate synthase is achieved by a mutation and/or overexpression of a gene coding for the enzyme having the function of the carbamoylphosphate synthase.

3:

• The microorganism of claim 1, wherein the activity of the L-arginine:glycine amidinotransferase is increased by a mutation and/or overexpression of the at least one heterologous gene coding for the protein having the activity of the L-arginine:glycine amidinotransferase.

4:

• The microorganism of claim 1, having an improved ability to produce L-arginine compared to the wildtype microorganism.

5:

• The microorganism of claim 4, wherein an activity of an enzyme having a function of an argininosuccinate lyase is increased compared to a respective enzymic activity in the wildtype microorganism.

6:

• The microorganism of claim 4, wherein an activity of an enzyme having a function of an omithine carbamoyltransferase is increased compared to a respective enzymic activity in the wildtype microorganism.

7:

• The microorganism of claim 4, wherein an activity of an enzyme having a function of an argininosuccinate synthetase is increased compared to a respective enzymic activity in the wildtype microorganism.

8:

• The microorganism of claim 5, wherein the increased activity of the enzyme having the function of an argininosuccinate lyase is achieved by a mutation and/or an overexpression of a gene coding for the respective enzyme.

(canceled)

10:

• The microorganism of claim 4, wherein the argR gene coding for the arginine responsive repressor protein ArgR is attenuated or deleted.

11:

• The microorganism of claim 4, wherein at least one or more of a gene coding for an enzyme of a biosynthetic pathway of L-ornithine and L-arginine is overexpressed,
• wherein the at least one or more of the gene coding comprises gdh, argJ, argB, argC and/or argD) coding for a glutamate dehydrogenase, for an ornithine acetyltransferase, for an acetylglutamate kinase, for an acetylglutamylphosphate reductase, and for an acetylornithine aminotransferase, respectively.

12:

• The microorganism of claim 1, wherein the protein having the activity of an L-arginine:glycine amidinotransferase comprises an amino acid sequence which is at least 70% identical to the amino acid sequence according to SEQ ID NO:
• 2.

13:

• The microorganism of claim 1, wherein the protein having the activity of an L-arginine:glycine amidinotransferase comprises an amino acid sequence which is at least 70% identical to the amino acid sequence according to SEQ ID NO:
• 16.

14:

• The microorganism of claim 1, wherein the protein having the activity of an L-arginine:glycine amidinotransferase comprises an amino acid sequence which is at least 70% identical to the amino acid sequence according to SEQ ID NO:
• 24.

15:

• The microorganism of claim 1, wherein the protein having the activity of an L-arginine:glycine amidinotransferase comprises an amino acid sequence which is at least 70% identical to the amino acid sequence according to SEQ ID NO:
• 20.

16:

• The microorganism of claim 1, wherein the microorganism belongs to the genus Corynebacterium, to the genus Enterobacteriaceae or to the genus Pseudomonas.

17:

• The microorganism of claim 16, wherein the microorganism is Corynebacterium glutamicum.

18:

• The microorganism of claim 16, wherein the microorganism is Escherichia coli.

19:

• The microorganism of claim 16, wherein the microorganism is Pseudomonas putida.

20:

• A method for the fermentative production of guanidino acetic acid (GAA), comprising:
• cultivating the microorganism as defined in claim 1 in a fermentation medium, and
• accumulating GAA in the medium, to form a GAA containing fermentation broth.

21:

• The method of claim 20, further comprising:
• isolating GAA from the GAA containing fermentation broth.

-26. (canceled)

27:

• The microorganism of claim 7, wherein the increased activity of the enzyme having the function of an argininosuccinate synthetase is achieved by a mutation and/or an overexpression of a gene coding for the respective enzyme.