Modified Polyketide Synthase Enzymes and Uses Thereof
AssigneeBgene Genetics
InventorsMatthieu Gousse, Ricardo De Andrade, Alexia Chandor-Proust
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Abstract
The present invention is in the field of genetically modified enzyme and microorganism comprising such a modified enzyme for the production of raspberry ketone or zingerone. The modified enzyme is a polyketide synthase (PKS) issued or derived from a wild type PKS. The modified PKS has the capability to produce vanillylidene acetone from feruloyl-CoA and/or 4-hydroxybenzalacetone from 4-coumaroyl-CoA in a more effective way as compared to wild type BAS or wild type PmPKS, in particular in recombinant bacteria strains.
Claims16
A recombinant polyketide synthase (PKS) issued or derived from a wild type PKS from Piper methysticum (PmPKS), Elaeis guineensis (EgPKS), or Vitis vinifera (VvPKS), the recombinant PKS having a P37A substitution and/or a M64Q substitution as compared to the wild type PKS from Vitis vinifera, or the recombinant PKS having a P38A substitution, and/or a V322P substitution as compared to the wild type PKS from Piper methysticum, or the recombinant PKS having a P39A substitution as compared to the wild type PKS from Elaeis guineensis.
(canceled)
Dependencies are inferred from the claim text.