Dioxygenase mutants and use thereof in synthesizing (2s,3r)-3-hydroxypipecolic acid

An L-proline-3-hydroxylase mutant, wherein the L-proline-3-hydroxylase mutant is obtained by mutating arginine at position 97 and asparagine at position 43 of the L-proline-3-hydroxylase whose amino acid sequence is shown in SEQ ID NO. 1;

• the L-proline-3-hydroxylase mutant is obtained by mutating arginine at position 97 of the L-proline-3-hydroxylase whose amino acid sequence is shown in SEQ ID NO. 1 to methionine, and mutating asparagine at position 43 thereof to threonine at the same time;
• or
• the L-proline-3-hydroxylase mutant is obtained by mutating arginine at position 97 of the L-proline-3-hydroxylase whose amino acid sequence is shown in SEQ ID NO. 1 to methionine, and mutating asparagine at position 43 thereof to alanine at the same time;
• or
• the L-proline-3-hydroxylase mutant is obtained by mutating arginine at position 97 of the L-proline-3-hydroxylase whose amino acid sequence is shown in SEQ ID NO. 1 to methionine, and mutating asparagine at position 43 thereof to valine at the same time;
• or
• the L-proline-3-hydroxylase mutant is obtained by mutating arginine at position 97 of the L-proline-3-hydroxylase whose amino acid sequence is shown in SEQ ID NO. 1 to methionine, and mutating asparagine at position 43 thereof to serine at the same time;
• or
• the L-proline-3-hydroxylase mutant is obtained by mutating arginine at position 97 of the L-proline-3-hydroxylase whose amino acid sequence is shown in SEQ ID NO. 1 to methionine, and mutating asparagine at position 43 thereof to aspartic acid at the same time;
• or
• the L-proline-3-hydroxylase mutant is obtained by mutating arginine at position 97 of the L-proline-3-hydroxylase whose amino acid sequence is shown in SEQ ID NO. 1 to methionine, and mutating asparagine at position 43 thereof to lysine at the same time.