Catalyst-free surface functionalization and polymer grafting
AssigneeIllumina, Inc.
InventorsLorenzo Berti, Andrew A. Brown, Wayne N. George
Life of this patent
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Abstract
Some embodiments described herein relate to a substrate with a surface comprising a silane or a silane derivative covalently attached to optionally substituted cycloalkene or optionally substituted heterocycloalkene for direct conjugation with a functionalized molecule of interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a polynucleotide, or a protein. In some embodiments, the silane or silane derivative contains optionally substituted norbornene or norbornene derivatives. Method for preparing a functionalized surface and the use in DNA sequencing and other diagnostic applications are also disclosed.
Claims21
-42. (canceled)
A method of immobilizing a functionalized molecule comprising functional groups to a first surface of a substrate, the method comprising:
- applying silane or a silane derivative comprising a first plurality of unsaturated moieties selected from the group consisting of cycloalkenes, cycloalkynes, heterocycloalkenes, heterocycloalkynes, and optionally substituted variants and combinations thereof covalently attached thereto onto the first surface of the substrate;
- and
- covalently attaching the functionalized molecule to the silane or silane derivative by reacting the functional groups of the functionalized molecule with the first plurality of unsaturated moieties to form a coating layer.
Dependencies are inferred from the claim text.